APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX20791-PEP).
Product Note
This antibody will recognise only one of three reported isoforms (NP_006331.1).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-SMSSADVEVARF, from the C Terminus of the protein sequence according to NP_006331.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BAI1 associated protein 2 , BAP2 , FLAF3 , IRSP53
Cellular Localization
Cytoplasm , Cytoplasm, cytoskeleton
Background
The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]
Database
Research Area
DATA IMAGES
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GTX20791 IHC-P Image
IHC-P analysis of human cortex using GTX20791 BAIAP2 (isoform 3) antibody. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75μg/ml
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GTX20791 WB Image
WB analysis of HEK293 overexpressing human BAIAP2 with DYKDDDDK tag using GTX20791 BAIAP2 (isoform 3) antibody. Lane A : primary antibody Lane B : Mock-transfected HEK293 probed with primary antibody Lane C : Tag antibody Dilution : 1μg/ml Loading : 10μg protein in RIPA buffer
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REFERENCE
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REVIEW
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