Immunohistochemical analysis of paraffin-embedded human liver, bile duct, using BAK1(GTX100063) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BAK antibody [N1N2], N-term (GTX100063) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

BAK antibody [N1N2], N-term detects BAK protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
BAK stained by BAK antibody [N1N2], N-term (GTX100063) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min