BAK antibody [N1N2], N-term

Name: BAK antibody [N1N2], N-term
Brand: GeneTex
SKU: GTX100063
Price: 399.00 USD
Rating: 5 (1 reviews)

Anti-BAK antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX100063

Anti-BAK antibody [N1N2], N-term used in Western Blot (WB). GTX100063

Cat. No. GTX100063

Reference 8

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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View product citations for antibody GTX100063 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Bovine, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human BAK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240507

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 antagonist/killer 1 , BAK , BAK-LIKE , BCL2L7 , CDN1

Cellular Localization

Membrane

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq]

Database

Gene ID: 578 BAK1
UniProt: Q16611 BAK1

Research Area

DATA IMAGES

GTX100063 IHC-P Image

BAK antibody [N1N2], N-term detects BAK protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
BAK stained by BAK antibody [N1N2], N-term (GTX100063) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX100063 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BAK antibody [N1N2], N-term (GTX100063) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX100063 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human liver, bile duct, using BAK1(GTX100063) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

Application : WB

Reactivity : Human

Lee CW et al. Molecules 2022; 27 (2) : 373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.

Application : WB

Reactivity : Human

J Exp Med 2021; 218 (10) Enhanced cGAS-STING-dependent interferon signaling associated with mutations in ATAD3A.

Application : WB

Reactivity : Human

Chen YH et al. Mol Med Rep 2021; 23 (1)

Lee CW et al. J Cell Physiol 2018; (Epub) Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.

Application : WB

Reactivity : Human

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