GeneTex
United States (US)

BAK antibody [TC100]

Bak-antibody-TC100_WB_GTX10808-1_19011507_289.jpg

Cat No. GTX10808

Host Mouse
Clonality Monoclonal
Clone Name TC100
Isotype IgG2a
Application WB, IHC-Fr
Reactivity Human
APPLICATION

Application Note

IHC-Fr: Use at a concentration of 1 - 5 μg/ml. WB: Use at a concentration of 2 - 5 μg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 23.4 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

23 kDa. ( Note )

Positive Control

Tonsil.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.09% Sodium Azide; Constituents: 0.2% Gelatin, 50mM Sodium Phosphate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment (C-terminal transmembrane portion deleted) (Human).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Antagonist/Killer 1,Bak,Bak-Like,Bcl2L7,Cdn1,Bak1

Background

Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl2 family members, among others, play an important role.The Bcl2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion.

Database

Research Area

DATA IMAGES
Bak-antibody-TC100_WB_GTX10808-1_19011507_289.jpg

GTX10808 WB Image

Detection of human Bak by immunoblotting. Sample: Whole cell lysates from untreated HL-60 cells. Primary antibody: Anti-Bak (Ab-1) Mouse mAb (TC-100) (GTX10808) (2 µg/ml). Detection: Chemiluminescence.

Package List Price ($)
$ 299