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BASP1 antibody

Anti-BASP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131728
Anti-BASP1 antibody used in Western Blot (WB). GTX131728
Anti-BASP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131728
Anti-BASP1 antibody used in Western Blot (WB). GTX131728
Anti-BASP1 antibody used in Western Blot (WB). GTX131728

Cat No. GTX131728

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

293T , A431 , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Zebrafish, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BASP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

brain abundant membrane attached signal protein 1,CAP-23,CAP23,NAP-22,NAP22

Cellular Localization

Cell membrane , Cell projection , growth cone

Background

This gene encodes a membrane bound protein with several transient phosphorylation sites and PEST motifs. Conservation of proteins with PEST sequences among different species supports their functional significance. PEST sequences typically occur in proteins with high turnover rates. Immunological characteristics of this protein are species specific. This protein also undergoes N-terminal myristoylation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BASP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131728

GTX131728 IHC-P Image

BASP1 antibody detects BASP1 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
BASP1 antibody (GTX131728) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BASP1 antibody used in Western Blot (WB). GTX131728

GTX131728 WB Image

BASP1 antibody detects BASP1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BASP1 antibody (GTX131728) diluted at 1:1000.

Anti-BASP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX131728

GTX131728 IHC-P Image

BASP1 antibody detects BASP1 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BASP1 antibody (GTX131728) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BASP1 antibody used in Western Blot (WB). GTX131728

GTX131728 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with BASP1 antibody (GTX131728) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BASP1 antibody used in Western Blot (WB). GTX131728

GTX131728 WB Image

Mouse tissue extract (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with BASP1 antibody (GTX131728) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE
There are currently no references for BASP1 antibody (GTX131728). Be the first to share your publications with this product.
REVIEW

BASP1 antibody Cat No. GTX131728

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 08-Jan-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 25 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: PANC1, Lane 2: HEK293
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169