APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2
Predict Reactivity
Zebrafish, Bovine, Dog, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.43 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human BAT1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949719
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BAT1 antibody , D6S81E antibody , UAP56 antibody , DDX39B antibody , nuclear RNA helicase (DEAD family) antibody , 56 kDa U2AF65-associated protein antibody , ATP-dependent RNA helicase p47 antibody , spliceosome RNA helicase DDX39B antibody , HLA-B-associated transcript 1 protein antibody , spliceosome RNA helicase BAT1 antibody , DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B antibody
Cellular Localization
Nucleus
Background
This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]
Research Area
DATA IMAGES
![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/templates/default/js/lightbox/images/loading.gif "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
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GTX101475 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft , using UAP56(GTX101475) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101475 WB Image
BAT1 antibody detects BAT1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAT1 antibody (GTX101475) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101475 IHC-P Image
UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
UAP56 antibody [N1C2] (GTX101475) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101475 ICC/IF Image
BAT1 antibody [N1C2] detects BAT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BAT1 protein stained by BAT1 antibody [N1C2] (GTX101475) diluted at 1:500.
Red: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar = 10 μm.
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GTX101475 IHC-P Image
BAT1 antibody [N1C2] detects BAT1 protein at nucleus in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
BAT1 antibody [N1C2] (GTX101475) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101475 IHC-P Image
UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
UAP56 antibody [N1C2] (GTX101475) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101475 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25360769
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GTX101475 WB Image
The data was published in the journal PLoS One in 2014. PMID: 25360769
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REFERENCE
REVIEW
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![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/upload/website/prouct_img/small/GTX101475/GTX101475_IHC_w_23060100_388.webp "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
![Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475](/upload/website/prouct_img/small/GTX101475/GTX101475_40079_20141031_WB_w_23060100_317.webp "Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475")
![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/upload/website/prouct_img/small/GTX101475/GTX101475_40079_20150506_IHC_M_w_23060100_602.webp "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
![Anti-BAT1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101475](/upload/website/prouct_img/small/GTX101475/GTX101475_42438_20160525_IFA_w_23060100_582.webp "Anti-BAT1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101475")
![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/upload/website/prouct_img/small/GTX101475/GTX101475_42403_20160413_IHC-P_R_w_23060100_395.webp "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/upload/website/prouct_img/small/GTX101475/GTX101475_40079_20150506_IHC_M_2_w_23060100_209.webp "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
![Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475](/upload/website/prouct_img/small/GTX101475/GTX101475_20240411_citeab_WB_2866_24041502_912.webp "Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475")
![Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475](/upload/website/prouct_img/small/GTX101475/GTX101475_20240411_citeab_WB_2493_24041502_301.webp "Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475")
![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/upload/website/prouct_img/normal/GTX101475/GTX101475_IHC_w_23060100_388.webp "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
![Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475](/upload/website/prouct_img/normal/GTX101475/GTX101475_40079_20141031_WB_w_23060100_317.webp "Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475")
![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/upload/website/prouct_img/normal/GTX101475/GTX101475_40079_20150506_IHC_M_w_23060100_602.webp "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
![Anti-BAT1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101475](/upload/website/prouct_img/normal/GTX101475/GTX101475_42438_20160525_IFA_w_23060100_582.webp "Anti-BAT1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101475")
![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/upload/website/prouct_img/normal/GTX101475/GTX101475_42403_20160413_IHC-P_R_w_23060100_395.webp "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
![Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475](/upload/website/prouct_img/normal/GTX101475/GTX101475_40079_20150506_IHC_M_2_w_23060100_209.webp "Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475")
![Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475](/upload/website/prouct_img/normal/GTX101475/GTX101475_20240411_citeab_WB_2866_24041502_912.webp "Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475")
![Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475](/upload/website/prouct_img/normal/GTX101475/GTX101475_20240411_citeab_WB_2493_24041502_301.webp "Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475")