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BAT1 antibody [N1C2]

Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475
Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475
Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475
Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475
Anti-BAT1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101475
Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475

Cat No. GTX101475

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Zebrafish, Bovine, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UAP56. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BAT1 antibody, D6S81E antibody, UAP56 antibody, DDX39B antibody, nuclear RNA helicase (DEAD family) antibody, 56 kDa U2AF65-associated protein antibody, ATP-dependent RNA helicase p47 antibody, spliceosome RNA helicase DDX39B antibody, HLA-B-associated transcript 1 protein antibody, spliceosome RNA helicase BAT1 antibody, DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B antibody

Cellular Localization

Nucleus

Background

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA, and also plays an important role in mRNA export from the nucleus to the cytoplasm. A cluster of genes, BAT1-BAT5, is localized in the vicinity of the genes for TNF alpha and TNF beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475

GTX101475 IHC-P Image

BAT1 antibody [N1C2] detects BAT1 protein at nucleus in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
BAT1 antibody [N1C2] (GTX101475) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475

GTX101475 IHC-P Image

UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
UAP56 antibody [N1C2] (GTX101475) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475

GTX101475 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft , using UAP56(GTX101475) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BAT1 antibody [N1C2] used in Western Blot (WB). GTX101475

GTX101475 WB Image

BAT1 antibody detects BAT1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BAT1 antibody (GTX101475) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BAT1 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101475

GTX101475 ICC/IF Image

BAT1 antibody [N1C2] detects BAT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BAT1 protein stained by BAT1 antibody [N1C2] (GTX101475) diluted at 1:500.
Red: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Scale bar = 10 μm.

Anti-BAT1 antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX101475

GTX101475 IHC-P Image

UAP56 antibody [N1C2] detects UAP56 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
UAP56 antibody [N1C2] (GTX101475) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf