APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
3-5μg/ml |
Note :
IHC-P
Human Spleen shows nucleoli staining in splenocytes.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88511-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_004630.3; NP_542433.1). Reported variants representing identical protein: NP_542434.1, NP_001092004.1, NP_542433.1 (isoform b).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-PETNAPNHPSPAEY, from the internal region of the protein sequence according to NP_004630.3; NP_542433.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 associated athanogene 6 , BAG-6 , BAT3 , D6S52E , G3
Cellular Localization
Cytoplasm, cytosol , Nucleus
Background
This gene was first characterized as part of a cluster of genes located within the human major histocompatibility complex class III region. This gene encodes a nuclear protein that is cleaved by caspase 3 and is implicated in the control of apoptosis. In addition, the protein forms a complex with E1A binding protein p300 and is required for the acetylation of p53 in response to DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX88511 IHC-P Image
IHC-P analysis of human spleen using GTX88511 BAT3 antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.8μg/ml
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REFERENCE
There are currently no references for BAT3 antibody, Internal (GTX88511). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BAT3 antibody, Internal (GTX88511). Be the first to share your experience with this product.