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BATF antibody [HL1870]

Recombinant antibody Orthogonal
Anti-BATF antibody [HL1870] used in Western Blot (WB). GTX637619
Anti-BATF antibody [HL1870] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637619
Anti-BATF antibody [HL1870] used in Western Blot (WB). GTX637619

Cat. No. GTX637619

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1870

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all BATF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human BATF

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

basic leucine zipper ATF-like transcription factor , B-ATF , BATF1 , SFA-2 , SFA2

Cellular Localization

Nucleus,Cytoplasm

Background

The protein encoded by this gene is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BATF antibody [HL1870] used in Western Blot (WB). GTX637619

GTX637619 WB Image

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with BATF antibody [HL1870] (GTX637619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BATF antibody [HL1870] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637619

GTX637619 ICC/IF Image

BATF antibody [HL1870] detects BATF protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BATF stained by BATF antibody [HL1870] (GTX637619) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-BATF antibody [HL1870] used in Western Blot (WB). GTX637619

GTX637619 WB Image

Orthogonal

MCF-7 whole cell and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BATF antibody [HL1870] (GTX637619) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations