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BBOX1 antibody

Anti-BBOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103127
Anti-BBOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103127
Anti-BBOX1 antibody used in Western Blot (WB). GTX103127
Anti-BBOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103127
Anti-BBOX1 antibody used in Western Blot (WB). GTX103127

Cat No. GTX103127

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Molt-4 , mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BBOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

gamma-butyrobetaine hydroxylase 1 , BBH , BBOX , G-BBH , gamma-BBH

Cellular Localization

Cytoplasm

Background

This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BBOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103127

GTX103127 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using BBOX1(GTX103127) antibody at 1:500 dilution.

Anti-BBOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103127

GTX103127 IHC-P Image

BBOX1 antibody detects BBOX1 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BBOX1 antibody (GTX103127) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BBOX1 antibody used in Western Blot (WB). GTX103127

GTX103127 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX103127 diluted at 1:500

Anti-BBOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103127

GTX103127 IHC-P Image

BBOX1 antibody detects BBOX1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
BBOX1 antibody (GTX103127) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BBOX1 antibody used in Western Blot (WB). GTX103127

GTX103127 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
7.5% SDS PAGE
GTX103127 diluted at 1:500

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169