GeneTex

  • Country / Location Selection

United States (US)

BBOX1 antibody

Anti-BBOX1 antibody used in Western Blot (WB). GTX103127
Anti-BBOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103127
Anti-BBOX1 antibody used in Western Blot (WB). GTX103127
Anti-BBOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103127
Anti-BBOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103127

Cat. No. GTX103127

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all BBOX1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Mouse liver , Molt-4

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BBOX1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949721

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gamma-butyrobetaine hydroxylase 1 , BBH , BBOX , G-BBH , gamma-BBH

Cellular Localization

Cytoplasm

Background

This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BBOX1 antibody used in Western Blot (WB). GTX103127

GTX103127 WB Image

Sample(30 μg of whole cell lysate)
A:MOLT4(GTX27912)
7.5% SDS PAGE
GTX103127 diluted at 1:500

Anti-BBOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103127

GTX103127 IHC-P Image

BBOX1 antibody detects BBOX1 protein at cytoplasm on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BBOX1 antibody (GTX103127) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BBOX1 antibody used in Western Blot (WB). GTX103127

GTX103127 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX103127 diluted at 1:500

Anti-BBOX1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103127

GTX103127 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using BBOX1(GTX103127) antibody at 1:500 dilution.

Anti-BBOX1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103127

GTX103127 IHC-P Image

BBOX1 antibody detects BBOX1 protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
BBOX1 antibody (GTX103127) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for BBOX1 antibody (GTX103127). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for BBOX1 antibody (GTX103127). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169