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BCA1 antibody [HL2172]

Recombinant antibody
Anti-BCA1 antibody [HL2172] used in Western Blot (WB). GTX638176
Anti-BCA1 antibody [HL2172] used in IHC (Paraffin sections) (IHC-P). GTX638176
Anti-BCA1 antibody [HL2172] used in IHC (Paraffin sections) (IHC-P). GTX638176

Cat. No. GTX638176

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2172

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BCA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCA1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine ligand 13 , ANGIE , ANGIE2 , BCA-1 , BCA1 , BLC , BLR1L , SCYB13

Cellular Localization

Secreted

Background

B lymphocyte chemoattractant, independently cloned and named Angie, is an antimicrobial peptide and CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq, Oct 2014]

Database

Research Area

DATA IMAGES

Anti-BCA1 antibody [HL2172] used in Western Blot (WB). GTX638176

GTX638176 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [HL2172] (GTX638176) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCA1 antibody [HL2172] used in IHC (Paraffin sections) (IHC-P). GTX638176

GTX638176 IHC-P Image

BCA1 antibody [HL2172] detects BCA1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human Clear Cell Renal Cell Carcinoma (ccRCC).
BCA1 stained by BCA1 antibody [HL2172] (GTX638176) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min

Anti-BCA1 antibody [HL2172] used in IHC (Paraffin sections) (IHC-P). GTX638176

GTX638176 IHC-P Image

BCA1 antibody [HL2172] detects secreted BCA1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human spleen.
BCA1 stained by BCA1 antibody [HL2172] (GTX638176) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations