BCA1 antibody [N3C3]

Name: BCA1 antibody [N3C3]
Brand: GeneTex
SKU: GTX108471
Price: 399.00 USD
Rating: 5 (2 reviews)

Anti-BCA1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108471

Anti-BCA1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108471

Anti-BCA1 antibody [N3C3] used in Western Blot (WB). GTX108471

Cat. No. GTX108471

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all BCA1 products

View product citations for antibody GTX108471 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.31 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036696

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine ligand 13 , ANGIE , ANGIE2 , BCA-1 , BCA1 , BLC , BLR1L , SCYB13

Background

B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq]

Database

Gene ID: 10563 CXCL13
UniProt: O43927 CXCL13

Research Area

DATA IMAGES

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects BCA1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects BCA1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108471 ICC/IF Image

BCA1 antibody [N3C3] detects secreted BCA1 protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCA1 protein stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108471 IHC-P Image

GTX108471 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Chao CC et al. J Cell Mol Med 2021; 25 (19) : 9128-9140 CXC chemokine ligand-13 promotes metastasis via CXCR5-dependent signaling pathway in non-small cell lung cancer.

Application : WB

Reactivity : Human

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