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BCA1 antibody [N3C3]

Anti-BCA1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108471
Anti-BCA1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108471
Anti-BCA1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108471
Anti-BCA1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108471
Anti-BCA1 antibody [N3C3] used in Western Blot (WB). GTX108471

Cat No. GTX108471

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

CXCL13-transfected 293T

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036696

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

C-X-C motif chemokine ligand 13 , ANGIE , ANGIE2 , BCA-1 , BCA1 , BLC , BLR1L , SCYB13

Background

B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCA1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108471

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BCA1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108471

GTX108471 ICC/IF Image

BCA1 antibody [N3C3] detects secreted BCA1 protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCA1 protein stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-BCA1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108471

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BCA1 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX108471

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects BCA1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BCA1 antibody [N3C3] used in Western Blot (WB). GTX108471

GTX108471 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

BCA1 antibody [N3C3] Cat No. GTX108471

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169