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BCA1 antibody [N3C3]

GTX108471_42907_20170921_IHC-P_19011507_273.jpg
GTX108471_42935_20170929_IHC-P_19011507_983.jpg
GTX108471_42844_20170621_IFA_19011507_275.jpg
GTX108471_42935_20171124_WB_B_19011507_938.jpg

Cat No. GTX108471

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

13 kDa. ( Note )

Positive Control

CXCL13-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-X-C Motif Chemokine Ligand 13,Angie,Angie2,Bca-1,Bca1,Blc,Blr1L,Scyb13,Cxcl13

Background

B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR-1). It may therefore function in the homing of B lymphocytes to follicles. [provided by RefSeq]

Database

DATA IMAGES
GTX108471_42907_20170921_IHC-P_19011507_273.jpg

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects secreted BCA1 protein in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108471_42935_20170929_IHC-P_19011507_983.jpg

GTX108471 IHC-P Image

BCA1 antibody [N3C3] detects BCA1 protein at cytoplasm in human esophageal carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophageal carcinoma.
BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX108471_42844_20170621_IFA_19011507_275.jpg

GTX108471 ICC/IF Image

BCA1 antibody [N3C3] detects secreted BCA1 protein by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCA1 protein stained by BCA1 antibody [N3C3] (GTX108471) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX108471_42935_20171124_WB_B_19011507_938.jpg

GTX108471 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with BCA1 antibody [N3C3] (GTX108471) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

BCA1 antibody [N3C3] Cat No. GTX108471

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
Lane Description : Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169