GeneTex
United States (US)

BCAP31 antibody [CC-1]

GTX15044_251_ICC-IF_19011519_146.jpg
GTX15044_250_ICC-IF_19011519_627.jpg
GTX15044_249_ICC-IF_19011519_142.jpg
GTX15044_2035_IHC-Fr_19011519_619.jpg
GTX15044_2092_WB_19011519_613.jpg

Cat No. GTX15044

Host Rat
Clonality Monoclonal
Clone Name CC-1
Isotype IgG2a
Application WB, ICC/IF, IHC-Fr, IP, ELISA
Reactivity Human, Mouse, Bovine, Hamster, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,500 - 1:6,000
ICC/IF 1:200-1:1000
IHC-Fr 1:1000 - 1:10000
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Amino acid residues 230-246 of BAP31 protein.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B Cell Receptor Associated Protein 31,6C6-Ag,Bap31,Cdm,Ddch,Dxs1357E,Bcap31

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum-Golgi intermediate compartment membrane

Background

This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES
GTX15044_251_ICC-IF_19011519_146.jpg

GTX15044 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX15044 BCAP31 antibody [CC-1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:200 incubated overnight at 4°C

GTX15044_250_ICC-IF_19011519_627.jpg

GTX15044 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX15044 BCAP31 antibody [CC-1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:200 incubated overnight at 4°C

GTX15044_249_ICC-IF_19011519_142.jpg

GTX15044 ICC/IF Image

ICC/IF analysis of Hela cells using GTX15044 BCAP31 antibody [CC-1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:200 incubated overnight at 4°C

GTX15044_2035_IHC-Fr_19011519_619.jpg

GTX15044 IHC-Fr Image

IHC-Fr analysis of baboon adrenal gland tissue using GTX15044 BCAP31 antibody [CC-1].

GTX15044_2092_WB_19011519_613.jpg

GTX15044 WB Image

WB analysis of samples using GTX15044 BCAP31 antibody [CC-1].

Package List Price ($)
$ 319