GeneTex
United States (US)

BCAP31 antibody [CC-4]

GTX15045_2036_IHC-P_19011519_935.jpg
GTX15045_1490_WB_19011519_634.jpg
GTX15045_2093_WB_19011519_961.jpg

Cat No. GTX15045

Host Rat
Clonality Monoclonal
Clone Name CC-4
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS, IP, IHC
Reactivity Human, Bovine, Hamster, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,500
ICC/IF 1:200 - 1:6,400
IHC-P 1:200 - 1:6,400
FACS 1:100
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Specificity/Sensitivity

This antibody binds to a more proximal region of BAP31 (amino acids 123-229). This sequence is conserved in non-human primate, bovine and hamster species.
PROPERTIES

Form

Liquid

Buffer

Ascites, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Amino acid residues 123-229 of human BAP31.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

B Cell Receptor Associated Protein 31,6C6-Ag,Bap31,Cdm,Ddch,Dxs1357E,Bcap31

Cellular Localization

Endoplasmic reticulum membrane,Endoplasmic reticulum-Golgi intermediate compartment membrane

Background

This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES
GTX15045_2036_IHC-P_19011519_935.jpg

GTX15045 IHC-P Image

IHC-P analysis of baboon pituitary gland tissue using GTX15045 BCAP31 antibody [CC-4].

GTX15045_1490_WB_19011519_634.jpg

GTX15045 WB Image

WB analysis of membrane enriched extracts (30µg lysate) of COS-7 (Lane 1), Raji (Lane 2), Molt-4 (Lane 3), HeLa (Lane 4), MCF7 (Lane 5), K-562 (Lane6), Hep G2 (Lane 7), Jurkat (Lane 8), HT-1080 (Lane 9) and HEK-293 (Lane 10) using GTX15045 BCAP31 antibody [CC-4].
Dilution : 1:1500

GTX15045_2093_WB_19011519_961.jpg

GTX15045 WB Image

WB analysis of samples using GTX15045 BCAP31 antibody [CC-4].

Package List Price ($)
$ 319