APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 20 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Kidney Tissue Slide , HeLa Cell Lysate
Product Note
At least four isoforms of BCAR3 are known to exist; this antibody will only recognize the largest isoform. BCAR3 antibody is predicted to not cross-react with other BCAR proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
BCAR3 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BCAR3.The immunogen is located within amino acids 30 - 80 of BCAR3.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCAR3 adaptor protein, NSP family member , AND-34 , NSP2 , SH2D3B
Background
Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Database
Research Area
DATA IMAGES
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GTX17178 WB Image
WB analysis of HeLa cell lysate using GTX17178 BCAR3 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX17178 IHC-P Image
IHC-P analysis of human kidney tissue using GTX17178 BCAR3 antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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