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BCAS2 antibody

Anti-BCAS2 antibody used in Western Blot (WB). GTX115390
Anti-BCAS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115390
Anti-BCAS2 antibody used in Western Blot (WB). GTX115390
Anti-BCAS2 antibody used in Western Blot (WB). GTX115390
Anti-BCAS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115390

Cat. No. GTX115390

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BCAS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

MCF-7 , Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCAS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619734

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCAS2 pre-mRNA processing factor , DAM1 , SPF27 , Snt309

Cellular Localization

Nucleus , nucleolus

Background

Involved in mRNA splicing.

Database

Research Area

DATA IMAGES

Anti-BCAS2 antibody used in Western Blot (WB). GTX115390

GTX115390 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCAS2 antibody (GTX115390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCAS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115390

GTX115390 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BCAS2(GTX115390) antibody at 1:200 dilution.

Anti-BCAS2 antibody used in Western Blot (WB). GTX115390

GTX115390 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCAS2 antibody (GTX115390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCAS2 antibody used in Western Blot (WB). GTX115390

GTX115390 WB Image

Sample (30 ug of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX115390 diluted at 1:1000

Anti-BCAS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX115390

GTX115390 IHC-P Image

Immunohistochemical analysis of paraffin-embedded J5 xenograft, using BCAS2(GTX115390) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169