Immunohistochemical analysis of paraffin-embedded J5 xenograft, using BCAS2(GTX115390) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX115390 diluted at 1:1000

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCAS2 antibody (GTX115390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BCAS2(GTX115390) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCAS2 antibody (GTX115390) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.