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BCAT1 antibody

Anti-BCAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04696
Anti-BCAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04696
Anti-BCAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04696
Anti-BCAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04696
Anti-BCAT1 antibody used in Western Blot (WB). GTX04696

Cat. No. GTX04696

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all BCAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:50-1:200
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol

Preservative

0.01% thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-190 of human BCAT1 (NP_005495.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCAT1,BCATC,BCT1,ECA39,MECA39,PNAS121,PP18,branched chain amino acid transaminase 1

Background

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Anti-BCAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04696

GTX04696 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX04696 BCAT1 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-BCAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04696

GTX04696 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX04696 BCAT1 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-BCAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04696

GTX04696 ICC/IF Image

ICC/IF analysis of PC-12 cells using GTX04696 BCAT1 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-BCAT1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04696

GTX04696 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX04696 BCAT1 antibody.
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-BCAT1 antibody used in Western Blot (WB). GTX04696

GTX04696 WB Image

WB analysis of various samples using GTX04696 BCAT1 antibody.
Loading : 25 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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Package List Price ($)
$ 399