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BCAT2 antibody

Anti-BCAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104121
Anti-BCAT2 antibody used in Western Blot (WB). GTX104121
Anti-BCAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104121

Cat No. GTX104121

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

BCAT2-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCAT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

branched chain amino acid transaminase 2 , BCAM , BCATM , BCT2 , PP18

Cellular Localization

Isoform A: Mitochondrion , Isoform B: Cytoplasm

Background

This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BCAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104121

GTX104121 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2(GTX104121) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCAT2 antibody used in Western Blot (WB). GTX104121

GTX104121 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCAT2 antibody (GTX104121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCAT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX104121

GTX104121 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Ca922 xenograft, using BCAT2(GTX104121) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169