GeneTex

  • Country / Location Selection

United States (US)

BCCIP antibody

Anti-BCCIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113357
Anti-BCCIP antibody used in Western Blot (WB). GTX113357
Anti-BCCIP antibody used in IHC (Paraffin sections) (IHC-P). GTX113357
Anti-BCCIP antibody used in IHC (Paraffin sections) (IHC-P). GTX113357

Cat. No. GTX113357

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BCCIP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

A431 , HeLa

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCCIP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038148

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRCA2 and CDKN1A interacting protein , TOK-1 , TOK1

Cellular Localization

Nucleus

Background

This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCCIP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113357

GTX113357 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using BCCIP (GTX113357) antibody at 1:200 dilution.

Anti-BCCIP antibody used in Western Blot (WB). GTX113357

GTX113357 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hela
10% SDS PAGE
GTX113357 diluted at 1:1000

Anti-BCCIP antibody used in IHC (Paraffin sections) (IHC-P). GTX113357

GTX113357 IHC-P Image

BCCIP antibody detects BCCIP protein at nucleus on BT483 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded BT483 xenograft.
BCCIP antibody (GTX113357) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCCIP antibody used in IHC (Paraffin sections) (IHC-P). GTX113357

GTX113357 IHC-P Image

BCCIP antibody detects BCCIP protein at nucleus on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
BCCIP antibody (GTX113357) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for BCCIP antibody (GTX113357). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for BCCIP antibody (GTX113357). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169