GeneTex

  • Country / Location Selection

United States (US)

BCHE antibody [N1N3]

Anti-BCHE antibody [N1N3] used in Western Blot (WB). GTX101304
Anti-BCHE antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101304
Anti-BCHE antibody [N1N3] used in Western Blot (WB). GTX101304

Cat. No. GTX101304

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BCHE products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

Jurkat , Raji , human BCHE-transfected 293T

Predict Reactivity

Mouse, Rat, Bovine, Cat, Dog, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.27 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCHE. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949728

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

butyrylcholinesterase , BCHED , CHE1 , CHE2 , E1

Background

Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCHE antibody [N1N3] used in Western Blot (WB). GTX101304

GTX101304 WB Image

BCHE antibody detects BCHE protein by Western blot analysis. Non-transfected (-) and BCHE-transfected (+, ) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BCHE antibody (GTX101304) at a dilution of 1:5000.

Anti-BCHE antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101304

GTX101304 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using BCHE(GTX101304) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCHE antibody [N1N3] used in Western Blot (WB). GTX101304

GTX101304 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
7.5% SDS PAGE
GTX101304 diluted at 1:1000

REFERENCE

There are currently no references for BCHE antibody [N1N3] (GTX101304). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for BCHE antibody [N1N3] (GTX101304). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169