Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [C1C3] (GTX111382) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [C1C3] (GTX111382) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 μg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX111382 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

BCKDHA antibody [C1C3] detects BCKDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BCKDHA protein stained by BCKDHA antibody [C1C3] (GTX111382) diluted at 1:400.
Blue: Hoechst 33342 staining.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using BCKDHA(GTX111382) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min