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BCKDHA antibody [HL2405]

Recombinant antibody
Anti-BCKDHA antibody [HL2405] used in IHC (Paraffin sections) (IHC-P). GTX638622
Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622
Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622
Anti-BCKDHA antibody [HL2405] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638622
Anti-BCKDHA antibody [HL2405] used in IHC (Whole mount) (IHC-Wm). GTX638622

Cat. No. GTX638622

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2405

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all BCKDHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human BCKDHA.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

branched chain keto acid dehydrogenase E1, alpha polypeptide , BCKDE1A , MSU , MSUD1 , OVD1A

Cellular Localization

Mitochondrion matrix

Background

The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-BCKDHA antibody [HL2405] used in IHC (Paraffin sections) (IHC-P). GTX638622

GTX638622 IHC-P Image

BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622

GTX638622 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (GTX638622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDHA antibody [HL2405] used in Western Blot (WB). GTX638622

GTX638622 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (GTX638622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDHA antibody [HL2405] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638622

GTX638622 ICC/IF Image

BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-BCKDHA antibody [HL2405] used in IHC (Whole mount) (IHC-Wm). GTX638622

GTX638622 IHC-Wm Image

BCKDHA antibody [HL2405] detects BCKDHA protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations