BCKDHA antibody [HL2405]

BCKDHA antibody [HL2405] detects BCKDHA protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (GTX638622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2405] (GTX638622) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

BCKDHA antibody [HL2405] detects BCKDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCKDHA stained by BCKDHA antibody [HL2405] (GTX638622) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).