GeneTex
  • Country / Location Selection

United States (US)

BCKDHA antibody [HL2434]

Recombinant antibody
Anti-BCKDHA antibody [HL2434] used in Western Blot (WB). GTX638750
Anti-BCKDHA antibody [HL2434] used in Western Blot (WB). GTX638750
Anti-BCKDHA antibody [HL2434] used in IHC (Paraffin sections) (IHC-P). GTX638750
Anti-BCKDHA antibody [HL2434] used in Western Blot (WB). GTX638750

Cat. No. GTX638750

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2434

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human BCKDHA.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

branched chain keto acid dehydrogenase E1, alpha polypeptide , BCKDE1A , MSU , MSUD1 , OVD1A

Cellular Localization

Mitochondrion matrix

Background

The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-BCKDHA antibody [HL2434] used in Western Blot (WB). GTX638750

GTX638750 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2434] (GTX638750) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDHA antibody [HL2434] used in Western Blot (WB). GTX638750

GTX638750 WB Image

Whole zebrafish extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2434] (GTX638750) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDHA antibody [HL2434] used in IHC (Paraffin sections) (IHC-P). GTX638750

GTX638750 IHC-P Image

BCKDHA antibody [HL2434] detects BCKDHA protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
BCKDHA stained by BCKDHA antibody [HL2434] (GTX638750) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BCKDHA antibody [HL2434] used in Western Blot (WB). GTX638750

GTX638750 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDHA antibody [HL2434] (GTX638750) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for BCKDHA antibody [HL2434] (GTX638750). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for BCKDHA antibody [HL2434] (GTX638750). Be the first to share your experience with this product.
Package List Price ($)
$ 399
$ 169
Custom Conjugations