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BCKDHA antibody, N-term

Anti-BCKDHA antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX45109
Anti-BCKDHA antibody, N-term used in Western Blot (WB). GTX45109
Anti-BCKDHA antibody, N-term used in Western Blot (WB). GTX45109

Cat. No. GTX45109

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
50 μg ($409)
See all BCKDHA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

human placenta , MCF-7

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human BCKDHA

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

branched chain keto acid dehydrogenase E1, alpha polypeptide , BCKDE1A , MSU , MSUD1 , OVD1A

Cellular Localization

Mitochondrion matrix

Background

The branched-chain alpha-keto acid (BCAA) dehydrogenase (BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). This gene encodes the alpha subunit of the decarboxylase (E1) component. Mutations in this gene result in maple syrup urine disease, type IA. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-BCKDHA antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX45109

GTX45109 IHC-P Image

IHC-P analysis of human lung tissue using GTX45109 BCKDHA antibody at 1:100. Left to right:DAPI, BCKDHA, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer.

Anti-BCKDHA antibody, N-term used in Western Blot (WB). GTX45109

GTX45109 WB Image

WB analysis of human placenta tissue using GTX45109 BCKDHA antibody at 1μg/ml.

Anti-BCKDHA antibody, N-term used in Western Blot (WB). GTX45109

GTX45109 WB Image

WB analysis of MCF-7 cells using GTX45109 BCKDHA antibody at 0.2-1μg/ml.

REFERENCE

Application Reference

Paul A Roberson et al. Front Nutr 2020; 7 : 628405 LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409