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BCKDK antibody [HL2710]

Recombinant antibody KO/KD Orthogonal
Anti-BCKDK antibody [HL2710] used in Western Blot (WB). GTX639471
Anti-BCKDK antibody [HL2710] used in Western Blot (WB). GTX639471
Anti-BCKDK antibody [HL2710] used in IHC (Paraffin sections) (IHC-P). GTX639471
Anti-BCKDK antibody [HL2710] used in Western Blot (WB). GTX639471

Cat. No. GTX639471

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2710

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BCKDK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human BCKDK.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

branched chain ketoacid dehydrogenase kinase , BCKDKD , BDK

Cellular Localization

Mitochondrion matrix,Mitochondrion

Background

The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-BCKDK antibody [HL2710] used in Western Blot (WB). GTX639471

GTX639471 WB Image

KO/KD

Wild-type (WT) and BCKDK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDK antibody [HL2710] used in Western Blot (WB). GTX639471

GTX639471 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDK antibody [HL2710] used in IHC (Paraffin sections) (IHC-P). GTX639471

GTX639471 IHC-P Image

BCKDK antibody [HL2710] detects BCKDK protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BCKDK stained by BCKDK antibody [HL2710] (GTX639471) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BCKDK antibody [HL2710] used in Western Blot (WB). GTX639471

GTX639471 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-FALSE was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations