Wild-type (WT) and BCKDK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

BCKDK antibody [HL2710] detects BCKDK protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BCKDK stained by BCKDK antibody [HL2710] (GTX639471) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (GTX639471) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.