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BCKDK antibody [N1C2]

Anti-BCKDK antibody [N1C2] used in Western Blot (WB). GTX119413
Anti-BCKDK antibody [N1C2] used in Western Blot (WB). GTX119413
Anti-BCKDK antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119413
Anti-BCKDK antibody [N1C2] used in Western Blot (WB). GTX119413

Cat No. GTX119413

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)
See all BCKDK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , mouse brain , rat brain

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCKDK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10633018

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

branched chain ketoacid dehydrogenase kinase , BCKDKD , BDK

Cellular Localization

Mitochondrion matrix

Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Database

Research Area

DATA IMAGES

Anti-BCKDK antibody [N1C2] used in Western Blot (WB). GTX119413

GTX119413 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX119413 diluted at 1:1000

Anti-BCKDK antibody [N1C2] used in Western Blot (WB). GTX119413

GTX119413 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] (GTX119413) diluted at 1:1000.

Anti-BCKDK antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX119413

GTX119413 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BCKDK(GTX119413) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCKDK antibody [N1C2] used in Western Blot (WB). GTX119413

GTX119413 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX119413 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169