GeneTex
United States (US)

BCKDK antibody [N2C3]

Orthogonal
GTX104477_39785_20180216_WB_Fraction_19011506_481.jpg
GTX104477_39785_WB_M_brain_19011506_139.jpg
GTX104477_39785_IHC_19011506_575.jpg
GTX104477_39785_20160804_WB_R_kidney_19011506_627.jpg
GTX104477_39785_20160804_WB_R_brain_19011506_531.jpg

Cat No. GTX104477

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

MCF-7 , MCF-7 membrane extract , Mouse brain , rat brain , rat kidney

Predict Reactivity

Zebrafish, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.79mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCKDK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Branched Chain Ketoacid Dehydrogenase Kinase,Bckdkd,Bdk,Bckdk

Cellular Localization

Mitochondrion matrix

Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Database

Research Area

DATA IMAGES
GTX104477_39785_20180216_WB_Fraction_19011506_481.jpg

GTX104477 WB Image

Orthogonal

MCF-7 whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104477_39785_WB_M_brain_19011506_139.jpg

GTX104477 WB Image

Sample (20 µg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX104477 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104477_39785_IHC_19011506_575.jpg

GTX104477 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCKDK(GTX104477) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104477_39785_20160804_WB_R_kidney_19011506_627.jpg

GTX104477 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104477_39785_20160804_WB_R_brain_19011506_531.jpg

GTX104477 WB Image

Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169