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BCKDK antibody [N2C3]

KO/KD Orthogonal
Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477
Anti-BCKDK antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104477
Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477
Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477
Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477
Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477
Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477
Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477
Anti-BCKDK antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104477

Cat. No. GTX104477

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BCKDK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

Mouse brain , rat kidney , rat brain

Predict Reactivity

Zebrafish, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCKDK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949729

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

branched chain ketoacid dehydrogenase kinase , BCKDKD , BDK

Cellular Localization

Mitochondrion matrix

Background

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Database

Research Area

DATA IMAGES

Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477

GTX104477 WB Image

KO/KD

Wild-type (WT) and BCKDK knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDK antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX104477

GTX104477 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BCKDK(GTX104477) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477

GTX104477 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477

GTX104477 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477

GTX104477 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477

GTX104477 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477

GTX104477 WB Image

Orthogonal

MCF-7 whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDK antibody [N2C3] used in Western Blot (WB). GTX104477

GTX104477 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N2C3] (GTX104477) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCKDK antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104477

GTX104477 ICC/IF Image

BCKDK antibody [N2C3] detects BCKDK protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCKDK stained by BCKDK antibody [N2C3] (GTX104477) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169