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BCKDK antibody

Anti-BCKDK antibody used in Western Blot (WB). GTX32468
Anti-BCKDK antibody used in IHC (Paraffin sections) (IHC-P). GTX32468
Anti-BCKDK antibody used in IHC (Paraffin sections) (IHC-P). GTX32468
Anti-BCKDK antibody used in IHC (Paraffin sections) (IHC-P). GTX32468
Anti-BCKDK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32468

Cat. No. GTX32468

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399)
See all BCKDK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 153-412 of human BCKDK (NP_005872.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

branched chain ketoacid dehydrogenase kinase , BCKDKD , BDK

Cellular Localization

Mitochondrion matrix,Mitochondrion

Background

The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Database

Research Area

DATA IMAGES

Anti-BCKDK antibody used in Western Blot (WB). GTX32468

GTX32468 WB Image

WB analysis of 22Rv1 cell lysate using GTX32468 BCKDK antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BCKDK antibody used in IHC (Paraffin sections) (IHC-P). GTX32468

GTX32468 IHC-P Image

IHC-P analysis of human liver damage tissue using GTX32468 BCKDK antibody.
Dilution : 1:100

Anti-BCKDK antibody used in IHC (Paraffin sections) (IHC-P). GTX32468

GTX32468 IHC-P Image

IHC-P analysis of rat lung tissue using GTX32468 BCKDK antibody.
Dilution : 1:100

Anti-BCKDK antibody used in IHC (Paraffin sections) (IHC-P). GTX32468

GTX32468 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32468 BCKDK antibody.
Dilution : 1:100

Anti-BCKDK antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32468

GTX32468 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32468 BCKDK antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399