BCL10 antibody [BL10/411]

Cat No. GTX34423

Host	Mouse
Clonality	Monoclonal
Clone Name	BL10/411
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human
Package	100 μg ($429)

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Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	0.5-1ug/ml
ICC/IF	1-2ug/ml
IHC-P	0.5-1ug/ml for 30 minutes at RT
Note : IHC-P Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1;14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NFĪŗB activation in a NIK-dependent pathway. This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1;14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Human BCL10 recombinant protein (epitope aa122-168)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

B Cell Cll/Lymphoma 10 , Carmen , Ciper , Clap , Imd37 , C-E10 , Me10 , Bcl10

Background

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq, Jul 2008]

Database

Gene ID: 8915
UniProt: O95999

Research Area

DATA IMAGES

GTX34423 WB Image

Western Blot of HepG2 Cell Lysate using Bcl-10 Monoclonal Antibody (BL10/411)

GTX34423 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with BCL10 Monoclonal Antibody (BL10/411).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 429

Qty

BCL10 antibody [BL10/411]

Cat No. GTX34423

Host

Clonality

Clone Name

Isotype

Application

Reactivity