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BCL10 antibody [N1C3]

Orthogonal IP/MS
Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159
Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159
Anti-BCL10 antibody [N1C3] used in ChIP assay (ChIP assay). GTX109159
Anti-BCL10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109159
Anti-BCL10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109159
Anti-BCL10 antibody [N1C3] used in Immunoprecipitation (IP). GTX109159
Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159

Cat. No. GTX109159

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all BCL10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HepG2 , Molt-4 , Raji , NIH-3T3

Product Note

IP/MS validation was supported by references (PMID:30377394)

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human BCL10 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949730

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL10 immune signaling adaptor , CARMEN , CIPER , CLAP , IMD37 , c-E10 , mE10

Cellular Localization

Cytoplasm , perinuclear region

Background

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159

GTX109159 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody [N1C3] (GTX109159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159

GTX109159 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody [N1C3] (GTX109159) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-BCL10 antibody [N1C3] used in ChIP assay (ChIP assay). GTX109159

GTX109159 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with TNF-a (10 ng/ml for 30 min) and 5 μg of either control rabbit IgG or anti-BCL10 antibody. The precipitated DNA was detected by PCR with primer set targeting to IκBα.
ChIP experiment and primer designs are based on J Biol Chem. 2006 Jan 6;281(1):167-75.

Anti-BCL10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109159

GTX109159 IHC-P Image

BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109159

GTX109159 IHC-P Image

BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL10 antibody [N1C3] used in Immunoprecipitation (IP). GTX109159

GTX109159 IP Image

BCL10 antibody [N1C3] immunoprecipitates BCL10 protein in IP experiments.
IP samples: Raji whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody [N1C3] (GTX109159)
12 % SDS-PAGE
The immunoprecipitated BCL10 protein was detected by BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159

GTX109159 WB Image

Sample (30 ug of whole cell lysate)

A: NIH-3T3

12% SDS PAGE

BCL10 antibody

GTX109159 diluted at 1:1000

REFERENCE

Application Reference

Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169