GeneTex
  • Country / Location Selection

United States (US)

BCL10 antibody [N1C3]

Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159
Anti-BCL10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109159
Anti-BCL10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109159
Anti-BCL10 antibody [N1C3] used in Immunoprecipitation (IP). GTX109159
Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159
Anti-BCL10 antibody [N1C3] used in ChIP assay (ChIP assay). GTX109159

Cat No. GTX109159

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP, ChIP assay

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Product Note

IP/MS validation was supported by references (PMID:30377394)

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human BCL10 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949730

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL10 immune signaling adaptor , CARMEN , CIPER , CLAP , IMD37 , c-E10 , mE10

Cellular Localization

Cytoplasm , perinuclear region

Background

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159

GTX109159 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
12% SDS PAGE
GTX109159 diluted at 1:1000

Anti-BCL10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109159

GTX109159 IHC-P Image

BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL10 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109159

GTX109159 IHC-P Image

BCL10 antibody [N1C3] detects BCL10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL10 antibody [N1C3] used in Immunoprecipitation (IP). GTX109159

GTX109159 IP Image

BCL10 antibody [N1C3] immunoprecipitates BCL10 protein in IP experiments.
IP samples: Raji whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody [N1C3] (GTX109159)
12 % SDS-PAGE
The immunoprecipitated BCL10 protein was detected by BCL10 antibody [N1C3] (GTX109159) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-BCL10 antibody [N1C3] used in Western Blot (WB). GTX109159

GTX109159 WB Image

Sample (30 ug of whole cell lysate)

A: NIH-3T3

12% SDS PAGE

BCL10 antibody

GTX109159 diluted at 1:1000

Anti-BCL10 antibody [N1C3] used in ChIP assay (ChIP assay). GTX109159

GTX109159 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with TNF-a (10 ng/ml for 30 min) and 5 μg of either control rabbit IgG or anti-BCL10 antibody. The precipitated DNA was detected by PCR with primer set targeting to IκBα.
ChIP experiment and primer designs are based on J Biol Chem. 2006 Jan 6;281(1):167-75.

REFERENCE

REVIEW

There are currently no reviews for BCL10 antibody [N1C3] (GTX109159). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169