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BCL10 antibody

Anti-BCL10 antibody used in Western Blot (WB). GTX112744
Anti-BCL10 antibody used in Western Blot (WB). GTX112744
Anti-BCL10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112744
Anti-BCL10 antibody used in IHC (Paraffin sections) (IHC-P). GTX112744
Anti-BCL10 antibody used in Western Blot (WB). GTX112744
Anti-BCL10 antibody used in Immunoprecipitation (IP). GTX112744

Cat No. GTX112744

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HeLa , Raji , Neuro2A , GL261 , C8D30

Product Note

IP/MS validation was supported by references (PMID:30377407)

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCL10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176414

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL10 immune signaling adaptor , CARMEN , CIPER , CLAP , IMD37 , c-E10 , mE10

Cellular Localization

Cytoplasm , perinuclear region

Background

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCL10 antibody used in Western Blot (WB). GTX112744

GTX112744 WB Image

Wild-type (WT) and BCL10 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody (GTX112744) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCL10 antibody used in Western Blot (WB). GTX112744

GTX112744 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX112744 diluted at 1:1000

Anti-BCL10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112744

GTX112744 ICC/IF Image

BCL10 antibody detects BCL10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: BCL10 protein stained by BCL10 antibody (GTX112744) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-BCL10 antibody used in IHC (Paraffin sections) (IHC-P). GTX112744

GTX112744 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BCL10 antibody(GTX112744) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL10 antibody used in Western Blot (WB). GTX112744

GTX112744 WB Image

BCL10 antibody detects BCL10 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
12 % SDS-PAGE
BCL10 antibody (GTX112744) dilution: 1:1000

Anti-BCL10 antibody used in Immunoprecipitation (IP). GTX112744

GTX112744 IP Image

BCL10 antibody immunoprecipitates BCL10 protein in IP experiments.
IP samples: Raji whole cell extract
A. 40 μg Raji whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody (GTX112744)
12 % SDS-PAGE
The immunoprecipitated BCL10 protein was detected by BCL10 antibody (GTX112744) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169