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BCL10 antibody

Anti-BCL10 antibody used in Western Blot (WB). GTX112744
Anti-BCL10 antibody used in Western Blot (WB). GTX112744
Anti-BCL10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112744
Anti-BCL10 antibody used in IHC (Paraffin sections) (IHC-P). GTX112744
Anti-BCL10 antibody used in Western Blot (WB). GTX112744
Anti-BCL10 antibody used in Immunoprecipitation (IP). GTX112744

Cat No. GTX112744

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Positive Control

HeLa , Raji , Neuro2A , GL261 , C8D30

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377407)

Predict Reactivity

Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCL10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCL10 immune signaling adaptor,CARMEN,CIPER,CLAP,IMD37,c-E10,mE10

Cellular Localization

Cytoplasm , perinuclear region

Background

This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-BCL10 antibody used in Western Blot (WB). GTX112744

GTX112744 WB Image

Wild-type (WT) and BCL10 knockout (KO) HeLa cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL10 antibody (GTX112744) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCL10 antibody used in Western Blot (WB). GTX112744

GTX112744 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX112744 diluted at 1:1000

Anti-BCL10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112744

GTX112744 ICC/IF Image

BCL10 antibody detects BCL10 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: BCL10 protein stained by BCL10 antibody (GTX112744) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-BCL10 antibody used in IHC (Paraffin sections) (IHC-P). GTX112744

GTX112744 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BCL10 antibody(GTX112744) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL10 antibody used in Western Blot (WB). GTX112744

GTX112744 WB Image

BCL10 antibody detects BCL10 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
12 % SDS-PAGE
BCL10 antibody (GTX112744) dilution: 1:1000

Anti-BCL10 antibody used in Immunoprecipitation (IP). GTX112744

GTX112744 IP Image

BCL10 antibody immunoprecipitates BCL10 protein in IP experiments.
IP samples: Raji whole cell extract
A. 40 μg Raji whole cell extract
B. Control with 4 μg of preimmune Rabbit IgG
C. Immunoprecipitation of BCL10 protein by 4 μg BCL10 antibody (GTX112744)
12 % SDS-PAGE
The immunoprecipitated BCL10 protein was detected by BCL10 antibody (GTX112744) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169