APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml for 30 minutes at RT |
| Assay dependent |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human bcl-6 protein fragment (around aa256-389) (Exact sequence is proprietary)
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL5 , BCL6 , BCL6A , Bcell CLL/lymphoma 6 , LAZ3 , ZBTB27 , ZNF51 , BCL6 transcription repressor
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
Database
Research Area
DATA IMAGES
![Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434](/templates/default/js/lightbox/images/loading.gif "Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434")
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GTX34434 IHC-P Image
IHC-P analysis of human follicular lymphoma tissue using GTX34434 BCL6 antibody [BCL6/1527].
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GTX34434 IHC-P Image
IHC-P analysis of human Hodgkin's lymphoma tissue using GTX34434 BCL6 antibody [BCL6/1527].
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GTX34434 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using bcl-6 Mouse Monoclonal Antibody (BCL6/1527). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX34434 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX34434 BCL6 antibody [BCL6/1527].
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REFERENCE
There are currently no references for BCL6 antibody [BCL6/1527] (GTX34434). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BCL6 antibody [BCL6/1527] (GTX34434). Be the first to share your experience with this product.
![Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434](/upload/website/prouct_img/small/GTX34434/GTX34434_20200115_IHC-P_577_w_23060801_304.webp "Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434")
![Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434](/upload/website/prouct_img/small/GTX34434/GTX34434_20200115_IHC-P_631_w_23060801_207.webp "Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434")
![Anti-BCL6 antibody [BCL6/1527] used in Protein Array (Protein Array). GTX34434](/upload/website/prouct_img/small/GTX34434/GTX34434_20200115_Protein-Array_1972_w_23060801_651.webp "Anti-BCL6 antibody [BCL6/1527] used in Protein Array (Protein Array). GTX34434")
![Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434](/upload/website/prouct_img/small/GTX34434/GTX34434_20200115_IHC-P_1266_w_23060801_432.webp "Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434")
![Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434](/upload/website/prouct_img/normal/GTX34434/GTX34434_20200115_IHC-P_577_w_23060801_304.webp "Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434")
![Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434](/upload/website/prouct_img/normal/GTX34434/GTX34434_20200115_IHC-P_631_w_23060801_207.webp "Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434")
![Anti-BCL6 antibody [BCL6/1527] used in Protein Array (Protein Array). GTX34434](/upload/website/prouct_img/normal/GTX34434/GTX34434_20200115_Protein-Array_1972_w_23060801_651.webp "Anti-BCL6 antibody [BCL6/1527] used in Protein Array (Protein Array). GTX34434")
![Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434](/upload/website/prouct_img/normal/GTX34434/GTX34434_20200115_IHC-P_1266_w_23060801_432.webp "Anti-BCL6 antibody [BCL6/1527] used in IHC (Paraffin sections) (IHC-P). GTX34434")