APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:60 |
|
Note :
IHC-P
Heat Induced Epitope Retreival (HIER) was performed in Epitope Retrieval Solution pH 9. The use of H₂O₂ to block endogenous peroxidase has a detrimental effect on the epitope recognized by this antibody. Solutions containing 1% or greater H₂O₂ can dramatically reduce the level of staining achievable. Blocking after unmasking can also lead to negative results. Therefore, it is recommended that endogenous peroxidase should be blocked before unmasking with 0.5% H₂O₂/methanol for 10 minutes. If this recommendation is not followed, staining intensity may be reduced.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Tissue culture supernatant
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Antigen Species
Human
Immunogen
Prokaryotic recombinant protein corresponding to 1–350 amino acids of the N-terminus of the Bcl-6 human oncoprotein molecule.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL6 transcription repressor , BCL5 , BCL6A , LAZ3 , ZBTB27 , ZNF51
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
Database
Research Area
DATA IMAGES
![Anti-BCL6 antibody [LN22] used in IHC (Paraffin sections) (IHC-P). GTX01937](/templates/default/js/lightbox/images/loading.gif "Anti-BCL6 antibody [LN22] used in IHC (Paraffin sections) (IHC-P). GTX01937")
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GTX01937 IHC-P Image
IHC-P analysis of human diffuse large B cell lymphoma using GTX01937 BCL6 antibody [LN22]. The majority of neoplastic cells show a moderate to strong nuclear staining reaction.
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REFERENCE
There are currently no references for BCL6 antibody [LN22] (GTX01937). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BCL6 antibody [LN22] (GTX01937). Be the first to share your experience with this product.
![Anti-BCL6 antibody [LN22] used in IHC (Paraffin sections) (IHC-P). GTX01937](/upload/website/prouct_img/small/GTX01937/GTX01937_20200811_IHC-P_105_w_23053121_903.webp "Anti-BCL6 antibody [LN22] used in IHC (Paraffin sections) (IHC-P). GTX01937")
![Anti-BCL6 antibody [LN22] used in IHC (Paraffin sections) (IHC-P). GTX01937](/upload/website/prouct_img/normal/GTX01937/GTX01937_20200811_IHC-P_105_w_23053121_903.webp "Anti-BCL6 antibody [LN22] used in IHC (Paraffin sections) (IHC-P). GTX01937")