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BCL7A antibody [C2C3], C-term

Anti-BCL7A antibody [C2C3], C-term used in Western Blot (WB). GTX100197
Anti-BCL7A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100197
Anti-BCL7A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100197
Anti-BCL7A antibody [C2C3], C-term used in Western Blot (WB). GTX100197
Anti-BCL7A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100197
Anti-BCL7A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100197
Anti-BCL7A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100197

Cat. No. GTX100197

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BCL7A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

Neuro2A , GL261 , HeLaS3

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCL7A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240513

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BAF chromatin remodeling complex subunit BCL7A , BCL7

Background

This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCL7A antibody [C2C3], C-term used in Western Blot (WB). GTX100197

GTX100197 WB Image

BCL7A antibody [C2C3], C-term detects BCL7A protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
12 % SDS-PAGE
BCL7A antibody [C2C3], C-term (GTX100197) dilution: 1:1000

Anti-BCL7A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100197

GTX100197 IHC-P Image

BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
BCL7A antibody [C2C3], C-term (GTX100197) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL7A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100197

GTX100197 ICC/IF Image

BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCL7A protein stained by BCL7A antibody [C2C3], C-term (GTX100197) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-BCL7A antibody [C2C3], C-term used in Western Blot (WB). GTX100197

GTX100197 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
15% SDS PAGE
GTX100197 diluted at 1:1000

Anti-BCL7A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100197

GTX100197 IHC-P Image

Immunohistochemical analysis of paraffin-embedded oral CA cell lines Ca922 xenograft, using BCL7A(GTX100197) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL7A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100197

GTX100197 IHC-P Image

BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
BCL7A antibody [C2C3], C-term (GTX100197) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BCL7A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100197

GTX100197 IHC-P Image

BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
BCL7A antibody [C2C3], C-term (GTX100197) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf
Package List Price ($)
$ 399
$ 169