GeneTex
United States (US)

BCL7A antibody [C2C3], C-term

GTX100197_39274_IHC_R_19011506_998.jpg
GTX100197_39274_WB_M_19011506_567.jpg
GTX100197_39274_IFA_19011506_597.jpg
GTX100197_39274_IHC_M_2_19011506_802.jpg
GTX100197_IHC_19011506_554.jpg
GTX100197_WB_19011506_806.jpg
GTX100197_39274_IHC_M_19011506_584.jpg

Cat No. GTX100197

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Positive Control

HeLaS3 , Neuro2A , GL261
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human BCL7A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCL7 antibody, BCL7A antibody, B-cell CLL/lymphoma 7 protein family member A antibody, B-cell CLL/lymphoma-7 antibody, B-cell CLL/lymphoma 7A antibody

Background

This gene is directly involved, with Myc and IgH, in a three-way gene translocation in a Burkitt lymphoma cell line. As a result of the gene translocation, the N-terminal region of the gene product is disrupted, which is thought to be related to the pathogenesis of a subset of high-grade B cell non-Hodgkin lymphoma. The N-terminal segment involved in the translocation includes the region that shares a strong sequence similarity with those of BCL7B and BCL7C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100197_39274_IHC_R_19011506_998.jpg

GTX100197 IHC-P Image

BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
BCL7A antibody [C2C3], C-term (GTX100197) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100197_39274_WB_M_19011506_567.jpg

GTX100197 WB Image

BCL7A antibody [C2C3], C-term detects BCL7A protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
12 % SDS-PAGE
BCL7A antibody [C2C3], C-term (GTX100197) dilution: 1:1000

GTX100197_39274_IFA_19011506_597.jpg

GTX100197 ICC/IF Image

BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCL7A protein stained by BCL7A antibody [C2C3], C-term (GTX100197) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX100197_39274_IHC_M_2_19011506_802.jpg

GTX100197 IHC-P Image

BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
BCL7A antibody [C2C3], C-term (GTX100197) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100197_IHC_19011506_554.jpg

GTX100197 IHC-P Image

Immunohistochemical analysis of paraffin-embedded oral CA cell lines Ca922 xenograft, using BCL7A(GTX100197) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100197_WB_19011506_806.jpg

GTX100197 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
15% SDS PAGE
GTX100197 diluted at 1:1000

GTX100197_39274_IHC_M_19011506_584.jpg

GTX100197 IHC-P Image

BCL7A antibody [C2C3], C-term detects BCL7A protein at nucleus on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
BCL7A antibody [C2C3], C-term (GTX100197) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Document
Unmodified.pdf