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BCL7C antibody

Orthogonal
Anti-BCL7C antibody used in Western Blot (WB). GTX115214
Anti-BCL7C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115214
Anti-BCL7C antibody used in IHC (Paraffin sections) (IHC-P). GTX115214

Cat. No. GTX115214

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($419),
25 μl ($169)
See all BCL7C products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCL7C. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11161872

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BAF chromatin remodeling complex subunit BCL7C

Background

This gene is identified by the similarity of its product to the N-terminal region of BCL7A protein. The BCL7A protein is encoded by the gene known to be directly involved in a three-way gene translocation in a Burkitt lymphoma cell line. The function of this gene has not yet been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BCL7C antibody used in Western Blot (WB). GTX115214

GTX115214 WB Image

Orthogonal

Jurkat whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BCL7C antibody (GTX115214) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BCL7C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115214

GTX115214 ICC/IF Image

BCL7C antibody detects BCL7C protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BCL7C protein stained by BCL7C antibody (GTX115214) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-BCL7C antibody used in IHC (Paraffin sections) (IHC-P). GTX115214

GTX115214 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using BCL7C(GTX115214) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169