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BCS1L antibody

Anti-BCS1L antibody used in Western Blot (WB). GTX32469
Anti-BCS1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32469
Anti-BCS1L antibody used in IHC (Paraffin sections) (IHC-P). GTX32469
Anti-BCS1L antibody used in IHC (Paraffin sections) (IHC-P). GTX32469

Cat. No. GTX32469

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all BCS1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 33-240 of human BCS1L (NP_004319.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCS1 homolog, ubiquinol-cytochrome c reductase complex chaperone , BCS , BCS1 , BJS , FLNMS , GRACILE , Hs.6719 , MC3DN1 , PTD , h-BCS , h-BCS1

Cellular Localization

Mitochondrion inner membrane

Background

This gene encodes a homolog of the S. cerevisiae bcs1 protein which is involved in the assembly of complex III of the mitochondrial respiratory chain. The encoded protein does not contain a mitochondrial targeting sequence but experimental studies confirm that it is imported into mitochondria. Mutations in this gene are associated with mitochondrial complex III deficiency and the GRACILE syndrome. Several alternatively spliced transcripts encoding two different isoforms have been described. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-BCS1L antibody used in Western Blot (WB). GTX32469

GTX32469 WB Image

WB analysis of various sample lysates using GTX32469 BCS1L antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BCS1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32469

GTX32469 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32469 BCS1L antibody.
Blue : DAPI
Dilution : 1:100

Anti-BCS1L antibody used in IHC (Paraffin sections) (IHC-P). GTX32469

GTX32469 IHC-P Image

IHC-P analysis of rat brain tissue using GTX32469 BCS1L antibody.
Dilution : 1:100

Anti-BCS1L antibody used in IHC (Paraffin sections) (IHC-P). GTX32469

GTX32469 IHC-P Image

IHC-P analysis of human stomach tissue using GTX32469 BCS1L antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399