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BDH2 antibody [4G4]

Overexpression
Anti-BDH2 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84833
Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833
Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833
Anti-BDH2 antibody [4G4] used in Western Blot (WB). GTX84833
Anti-BDH2 antibody [4G4] used in Western Blot (WB). GTX84833
Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833
Anti-BDH2 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX84833

Cat. No. GTX84833

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4G4

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all BDH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BDH2(NP_064524) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

3-hydroxybutyrate dehydrogenase 2 , DHRS6 , EFA6R , PRO20933 , SDR15C1 , UCPA-OR , UNQ6308

Cellular Localization

Cytoplasm

Background

Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase.

Database

Research Area

DATA IMAGES

Anti-BDH2 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84833

GTX84833 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with BDH2 plasmid using GTX84833 BDH2 antibody [4G4].

Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833

GTX84833 FACS Image

FACS analysis of HeLa cells using GTX84833 BDH2 antibody [4G4].
Red : Primary antibody
Blue : Negative control antibody

Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833

GTX84833 FACS Image

FACS analysis of Jurkat cells using GTX84833 BDH2 antibody [4G4].
Red : Primary antibody
Blue : Negative control antibody

Anti-BDH2 antibody [4G4] used in Western Blot (WB). GTX84833

GTX84833 WB Image

Overexpression

WB analysis of HEK293T cells transfected with BDH2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84833 BDH2 antibody [4G4].
Loading : 5 ug per lane

Anti-BDH2 antibody [4G4] used in Western Blot (WB). GTX84833

GTX84833 WB Image

WB analysis of various cell lines using GTX84833 BDH2 antibody [4G4].
Loading : 10 ug per lane
Dilution : 1:200

Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833

GTX84833 FACS Image

FACS analysis of HEK293T cells transfected with either BDH2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84833 BDH2 antibody [4G4].

Anti-BDH2 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX84833

GTX84833 IHC-P Image

IHC-P analysis of human liver tissue using GTX84833 BDH2 antibody [4G4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519