GeneTex
United States (US)

BDH2 antibody [4G4]

Anti-BDH2 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX84833
Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833
Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833
Anti-BDH2 antibody [4G4] used in Western Blot (WB). GTX84833
Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833
Anti-BDH2 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84833
Anti-BDH2 antibody [4G4] used in Western Blot (WB). GTX84833

Cat No. GTX84833

Host Mouse
Clonality Monoclonal
Clone Name 4G4
Isotype IgG2a
Application WB, ICC/IF, IHC-P, FACS
Reactivity Human, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

27 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.73 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BDH2(NP_064524) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

3-Hydroxybutyrate Dehydrogenase 2,Dhrs6,Efa6R,Pro20933,Sdr15C1,Ucpa-Or,Unq6308,Bdh2

Cellular Localization

Cytoplasm

Background

Dehydrogenase that mediates the formation of 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase.

Database

Research Area

DATA IMAGES
Anti-BDH2 antibody [4G4] used in IHC (Paraffin sections) (IHC-P). GTX84833

GTX84833 IHC-P Image

IHC-P analysis of human liver tissue using GTX84833 BDH2 antibody [4G4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100℃ for 10min.
Dilution : 1:50

Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833

GTX84833 FACS Image

FACS analysis of Jurkat cells using GTX84833 BDH2 antibody [4G4].
Red : Primary antibody
Blue : Negative control antibody

Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833

GTX84833 FACS Image

FACS analysis of HeLa cells using GTX84833 BDH2 antibody [4G4].
Red : Primary antibody
Blue : Negative control antibody

Anti-BDH2 antibody [4G4] used in Western Blot (WB). GTX84833

GTX84833 WB Image

WB analysis of various cell lines using GTX84833 BDH2 antibody [4G4].
Loading : 10 ug per lane
Dilution : 1:200

Anti-BDH2 antibody [4G4] used in Flow cytometry (FACS). GTX84833

GTX84833 FACS Image

FACS analysis of HEK293T cells transfected with either BDH2 plasmid(Red) or empty vector control plasmid(Blue) using GTX84833 BDH2 antibody [4G4].

Anti-BDH2 antibody [4G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84833

GTX84833 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with BDH2 plasmid using GTX84833 BDH2 antibody [4G4].

Anti-BDH2 antibody [4G4] used in Western Blot (WB). GTX84833

GTX84833 WB Image

WB analysis of HEK293T cells transfected with BDH2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84833 BDH2 antibody [4G4].
Loading : 5 ug per lane

Package List Price ($)
$ 339