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BDNF antibody

Anti-BDNF antibody used in Western Blot (WB). GTX134514
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX134514
Anti-BDNF antibody used in Western Blot (WB). GTX134514
Anti-BDNF antibody used in Western Blot (WB). GTX134514
Anti-BDNF antibody used in Western Blot (WB). GTX134514

Cat No. GTX134514

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brian , Mouse lung , rat brain , DDDDK-tagged BDNF-transfected 293T

Predict Reactivity

Zebrafish, Bovine, Cat, Dog, Chicken, Pig, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse BDNF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887301

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

brain derived neurotrophic factor

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-BDNF antibody used in Western Blot (WB). GTX134514

GTX134514 WB Image

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX134514) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX134514

GTX134514 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: BDNF stained by BDNF antibody (GTX134514) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in Western Blot (WB). GTX134514

GTX134514 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX134514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in Western Blot (WB). GTX134514

GTX134514 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX134514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-BDNF antibody used in Western Blot (WB). GTX134514

GTX134514 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (0 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX134514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169