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BDNF antibody

Orthogonal Overexpression
Anti-BDNF antibody used in Western Blot (WB). GTX134514
Anti-BDNF antibody used in Western Blot (WB). GTX134514
Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX134514
Anti-BDNF antibody used in Western Blot (WB). GTX134514
Anti-BDNF antibody used in Western Blot (WB). GTX134514

Cat. No. GTX134514

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BDNF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

DDDDK-tagged BDNF-transfected 293T , mouse brian

Predict Reactivity

Zebrafish, Bovine, Cat, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse BDNF. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887301

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

brain derived neurotrophic factor

Cellular Localization

Secreted

Background

The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]

Database

Research Area

DATA IMAGES

Anti-BDNF antibody used in Western Blot (WB). GTX134514

GTX134514 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX134514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in Western Blot (WB). GTX134514

GTX134514 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (0 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX134514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in IHC (Paraffin sections) (IHC-P). GTX134514

GTX134514 IHC-P Image

BDNF antibody detects BDNF protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: BDNF stained by BDNF antibody (GTX134514) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BDNF antibody used in Western Blot (WB). GTX134514

GTX134514 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX134514) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BDNF antibody used in Western Blot (WB). GTX134514

GTX134514 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with BDNF antibody (GTX134514) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

Application Reference

Caputi FF et al. Drug Alcohol Depend 2019; 197 : 127-133 Short-term withdrawal from repeated exposure to cocaine during adolescence modulates dynorphin mRNA levels and BDNF signaling in the rat nucleus accumbens.
Application : WB
Reactivity : Rat
Wei CL et al. Brain Res 2019; (Epub) Antidepressant-like activities of live and heat-killed Lactobacillus paracasei PS23 in chronic corticosterone-treated mice and possible mechanisms.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169