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BHMT antibody [3D6]

Anti-BHMT antibody [3D6] used in Western Blot (WB). GTX50061

Cat. No. GTX50061

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D6

Isotype

 IgG2a

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all BHMT products

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human BHMT (1-406aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

betaine--homocysteine S-methyltransferase , BHMT1 , HEL-S-61p

Cellular Localization

Cytoplasm

Background

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BHMT antibody [3D6] used in Western Blot (WB). GTX50061

GTX50061 WB Image

The extracts of mouse liver(each 20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BHMT (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399