BHMT antibody [3E11]

Cat. No. GTX84828

Host	Mouse
Clonality	Monoclonal
Clone Name	3E11
Isotype	IgG2a
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μl ($519)

See all BHMT products

Datasheet
Document

View product citations for antibody GTX84828 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:200
ICC/IF	Assay dependent
IHC-P	1:200
FACS	Assay dependent

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BHMT (NP_001704) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

betaine--homocysteine S-methyltransferase , BHMT1 , HEL-S-61p

Cellular Localization

Cytoplasm

Background

Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.

Database

Gene ID: 635 BHMT
UniProt: Q93088 BHMT

Research Area

DATA IMAGES

GTX84828 WB Image

WB analysis of HepG2 cell lysate using GTX84828 BHMT antibody [3E11].
Loading : 10 ug per lane
Dilution : 1:200

GTX84828 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84828 BHMT antibody [3E11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84828 WB Image

WB analysis of HepG2 cell lysate using GTX84828 BHMT antibody [3E11].
Loading : 10 ug per lane
Dilution : 1:200

GTX84828 FACS Image

FACS analysis of HEK293T cells transfected with either BHMT plasmid(Red) or empty vector control plasmid(Blue) using GTX84828 BHMT antibody [3E11].

GTX84828 IHC-P Image

IHC-P analysis of liver tissue using GTX84828 BHMT antibody [3E11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84828 IHC-P Image

IHC-P analysis of kidney tissue using GTX84828 BHMT antibody [3E11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

BHMT antibody [3E11]

Cat. No. GTX84828

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84828 WB Image

GTX84828 IHC-P Image

GTX84828 WB Image

GTX84828 FACS Image

GTX84828 IHC-P Image

GTX84828 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.