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BHMT antibody [N3C3]

Anti-BHMT antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102983
Anti-BHMT antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102983
Anti-BHMT antibody [N3C3] used in Western Blot (WB). GTX102983
Anti-BHMT antibody [N3C3] used in Western Blot (WB). GTX102983

Cat. No. GTX102983

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all BHMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BHMT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949741

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

betaine--homocysteine S-methyltransferase , BHMT1 , HEL-S-61p

Background

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BHMT antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102983

GTX102983 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HepG2, using BHMT(GTX102983) antibody at 1:200 dilution.

Anti-BHMT antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX102983

GTX102983 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SG xenograft , using BHMT(GTX102983) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BHMT antibody [N3C3] used in Western Blot (WB). GTX102983

GTX102983 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody (GTX102983) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 18498552

Anti-BHMT antibody [N3C3] used in Western Blot (WB). GTX102983

GTX102983 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody [N3C3] (GTX102983) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169