Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody [N3C3] (GTX102983) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody (GTX102983) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded SG xenograft , using BHMT(GTX102983) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HepG2, using BHMT(GTX102983) antibody at 1:200 dilution.