GeneTex
  • Country / Location Selection

United States (US)

BHMT antibody

Anti-BHMT antibody used in Western Blot (WB). GTX103037
Anti-BHMT antibody used in Western Blot (WB). GTX103037
Anti-BHMT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103037
Anti-BHMT antibody used in IHC (Paraffin sections) (IHC-P). GTX103037

Cat No. GTX103037

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BHMT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

betaine--homocysteine S-methyltransferase , BHMT1 , HEL-S-61p

Cellular Localization

Cytoplasm

Background

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BHMT antibody used in Western Blot (WB). GTX103037

GTX103037 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX103037 diluted at 1:1000

Anti-BHMT antibody used in Western Blot (WB). GTX103037

GTX103037 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody (GTX103037) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 18498552

Anti-BHMT antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103037

GTX103037 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using BHMT(GTX103037) antibody at 1:500 dilution.

Anti-BHMT antibody used in IHC (Paraffin sections) (IHC-P). GTX103037

GTX103037 IHC-P Image

BHMT antibody detects BHMT protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
BHMT antibody (GTX103037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for BHMT antibody (GTX103037). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for BHMT antibody (GTX103037). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169