GeneTex
United States (US)

BHMT antibody

GTX103037_WB_19011506_674.jpg
GTX103037_39967_20161117_WB_M_19011506_527.jpg
GTX103037_39967_IFA_19011506_617.jpg
GTX103037_39967_IHC_19011506_460.jpg

Cat No. GTX103037

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HepG2 , mouse liver

Predict Reactivity

Rat, Zebrafish, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BHMT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Betaine--Homocysteine S-Methyltransferase,Bhmt1,Hel-S-61P,Bhmt

Cellular Localization

Cytoplasm

Background

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Database

DATA IMAGES
GTX103037_WB_19011506_674.jpg

GTX103037 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX103037 diluted at 1:1000

GTX103037_39967_20161117_WB_M_19011506_527.jpg

GTX103037 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with BHMT antibody (GTX103037) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 18498552

GTX103037_39967_IFA_19011506_617.jpg

GTX103037 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using BHMT(GTX103037) antibody at 1:500 dilution.

GTX103037_39967_IHC_19011506_460.jpg

GTX103037 IHC-P Image

BHMT antibody detects BHMT protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
BHMT antibody (GTX103037) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169