GeneTex
United States (US)

BHMT2 antibody [N2C3]

GTX115754_40296_WB_M_liver_18121410_489.jpg
GTX115754_40296_IFA_18121410_672.jpg
GTX115754_40296_WB_18121410_668.jpg

Cat No. GTX115754

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

HCT116 , mouse liver

Predict Reactivity

Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BHMT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Betaine--Homocysteine S-Methyltransferase 2,Bhmt2

Background

Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. [provided by RefSeq]

Database

DATA IMAGES
GTX115754_40296_WB_M_liver_18121410_489.jpg

GTX115754 WB Image

Sample (20 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
GTX115754 diluted at 1:20000

GTX115754_40296_IFA_18121410_672.jpg

GTX115754 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using BHMT2(GTX115754) antibody at 1:200 dilution.

GTX115754_40296_WB_18121410_668.jpg

GTX115754 WB Image

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX115754 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169