APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , Rat2 , mouse lung
Predict Reactivity
Bovine, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human BICD2. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888272
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BICD cargo adaptor 2 , SMALED2 , SMALED2A , SMALED2B , bA526D8.1
Cellular Localization
Golgi apparatus , Cytoplasm , cytoskeleton
Background
This gene is one of two human homologs of Drosophila bicaudal-D and a member of the Bicoid family. It has been implicated in dynein-mediated, minus end-directed motility along microtubules. It has also been reported to be a phosphorylation target of NIMA related kinase 8. Two alternative splice variants have been described. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/templates/default/js/lightbox/images/loading.gif "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
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GTX631994 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] (GTX631994) diluted at 1:500.
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GTX631994 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] (GTX631994) diluted at 1:1000.
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GTX631994 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] (GTX631994) diluted at 1:1000.
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GTX631994 WB Image
BICD2 antibody detects BICD2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody (GTX631994) diluted by 1:1000.
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GTX631994 ICC/IF Image
BICD2 antibody [GT10811] detects BICD2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.
Green: BICD2 protein stained by BICD2 antibody [GT10811] (GTX631994) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX631994 IP Image
Immunoprecipitation of BICD2 protein from A431 whole cell extracts using 5 μg of BICD2 antibody [GT10811] (GTX631994).
Western blot analysis was performed using BICD2 antibody [GT10811] (GTX631994).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
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REFERENCE
There are currently no references for BICD2 antibody [GT10811] (GTX631994). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BICD2 antibody [GT10811] (GTX631994). Be the first to share your experience with this product.
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/upload/website/prouct_img/small/GTX631994/GTX631994_41911_20161117_WB_shRNA_watermark_w_23061202_373.webp "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/upload/website/prouct_img/small/GTX631994/GTX631994_41911_20160811_WB_R_w_23061202_227.webp "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/upload/website/prouct_img/small/GTX631994/GTX631994_41911_20160825_WB_M_lung_w_23061202_254.webp "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/upload/website/prouct_img/small/GTX631994/GTX631994_41911_20141128_WB_w_23061202_344.webp "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631994](/upload/website/prouct_img/small/GTX631994/GTX631994_41911_IFA_w_23061202_772.webp "Anti-BICD2 antibody [GT10811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Immunoprecipitation (IP). GTX631994](/upload/website/prouct_img/small/GTX631994/GTX631994_41911_20150710_IP_w_23061202_523.webp "Anti-BICD2 antibody [GT10811] used in Immunoprecipitation (IP). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/upload/website/prouct_img/normal/GTX631994/GTX631994_41911_20161117_WB_shRNA_watermark_w_23061202_373.webp "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/upload/website/prouct_img/normal/GTX631994/GTX631994_41911_20160811_WB_R_w_23061202_227.webp "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/upload/website/prouct_img/normal/GTX631994/GTX631994_41911_20160825_WB_M_lung_w_23061202_254.webp "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994](/upload/website/prouct_img/normal/GTX631994/GTX631994_41911_20141128_WB_w_23061202_344.webp "Anti-BICD2 antibody [GT10811] used in Western Blot (WB). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631994](/upload/website/prouct_img/normal/GTX631994/GTX631994_41911_IFA_w_23061202_772.webp "Anti-BICD2 antibody [GT10811] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX631994")
![Anti-BICD2 antibody [GT10811] used in Immunoprecipitation (IP). GTX631994](/upload/website/prouct_img/normal/GTX631994/GTX631994_41911_20150710_IP_w_23061202_523.webp "Anti-BICD2 antibody [GT10811] used in Immunoprecipitation (IP). GTX631994")