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BICD2 antibody [GT1824]

KO/KD
Anti-BICD2 antibody [GT1824] used in Western Blot (WB). GTX631995
Anti-BICD2 antibody [GT1824] used in Western Blot (WB). GTX631995
Anti-BICD2 antibody [GT1824] used in Western Blot (WB). GTX631995
Anti-BICD2 antibody [GT1824] used in Immunoprecipitation (IP). GTX631995

Cat. No. GTX631995

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1824

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all BICD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , PC-12 , Rat2

Predict Reactivity

Mouse, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BICD2. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888273

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BICD cargo adaptor 2 , SMALED2 , SMALED2A , SMALED2B , bA526D8.1

Cellular Localization

Golgi apparatus , Cytoplasm , cytoskeleton

Background

This gene is one of two human homologs of Drosophila bicaudal-D and a member of the Bicoid family. It has been implicated in dynein-mediated, minus end-directed motility along microtubules. It has also been reported to be a phosphorylation target of NIMA related kinase 8. Two alternative splice variants have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BICD2 antibody [GT1824] used in Western Blot (WB). GTX631995

GTX631995 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT1824] (GTX631995) diluted at 1:500.

Anti-BICD2 antibody [GT1824] used in Western Blot (WB). GTX631995

GTX631995 WB Image

BICD2 antibody detects BICD2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody (GTX631995) diluted by 1:1000.

Anti-BICD2 antibody [GT1824] used in Western Blot (WB). GTX631995

GTX631995 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT1824] (GTX631995) diluted at 1:500.

Anti-BICD2 antibody [GT1824] used in Immunoprecipitation (IP). GTX631995

GTX631995 IP Image

Immunoprecipitation of BICD2 protein from A431 whole cell extracts using 5 μg of BICD2 antibody [GT1824] (GTX631995).
Western blot analysis was performed using BICD2 antibody [GT1824] (GTX631995).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations