*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
The peptide used to generate this antibody is available for purchase (GTX88650-PEP).
This antibody is expected to recognise all ten reported isoforms (NP_647593.1, NP_647594.1, NP_647595.1, NP_647596.1, NP_647597.1, NP_647598.1, NP_647599.1, NP_004296.1, NP_647600.1, NP_647601.1).
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Peptide with sequence C-SDNAPAKGNKSPS, from the internal region of the protein sequence according to NP_004296.1; NP_647593.1; NP_647594.1; NP_647595.1; NP_647596.1; NP_647597.1; NP_647598.1; NP_647599.1; NP_647600.1; NP_647601.1.
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Bridging Integrator 1,Amph2,Amphl,Cnm2,Sh3P9,Bin1
This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq, Sep 2011]
GTX88650 WB Image
WB analysis of human skeletal muscle lysate using GTX88650 BIN1 antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer