GeneTex
United States (US)

BIN1 antibody, Internal

GTX88650_2188_WB_18121411_312.jpg

Cat No. GTX88650

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1-3μg/ml
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88650-PEP).

Specificity/Sensitivity

This antibody is expected to recognise all ten reported isoforms (NP_647593.1, NP_647594.1, NP_647595.1, NP_647596.1, NP_647597.1, NP_647598.1, NP_647599.1, NP_004296.1, NP_647600.1, NP_647601.1).

Predict Reactivity

Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SDNAPAKGNKSPS, from the internal region of the protein sequence according to NP_004296.1; NP_647593.1; NP_647594.1; NP_647595.1; NP_647596.1; NP_647597.1; NP_647598.1; NP_647599.1; NP_647600.1; NP_647601.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bridging Integrator 1,Amph2,Amphl,Cnm2,Sh3P9,Bin1

Background

This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynamin, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES
GTX88650_2188_WB_18121411_312.jpg

GTX88650 WB Image

WB analysis of human skeletal muscle lysate using GTX88650 BIN1 antibody, Internal.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer