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BLK antibody [1E6]

Anti-BLK antibody [1E6] used in Flow cytometry (FACS). GTX80405
Anti-BLK antibody [1E6] used in Western Blot (WB). GTX80405
Anti-BLK antibody [1E6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80405

Cat. No. GTX80405

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1E6

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all BLK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human BLK expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BLK proto-oncogene, Src family tyrosine kinase , MODY11

Cellular Localization

Cell membrane

Background

This gene encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-BLK antibody [1E6] used in Flow cytometry (FACS). GTX80405

GTX80405 FACS Image

FACS analysis of HL-60 cells using GTX80405 BLK antibody [1E6].
Green : BLK
Purple : negative control

Anti-BLK antibody [1E6] used in Western Blot (WB). GTX80405

GTX80405 WB Image

WB analysis of HEK293 (1) and BLK(AA: 2-200)-hIgGFc transfected HEK293 (2) cell lysate using GTX80405 BLK antibody [1E6].

Anti-BLK antibody [1E6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80405

GTX80405 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX80405 BLK antibody [1E6].
Green : BLK
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399