GeneTex
United States (US)

BLM antibody [C3], C-term

GTX101303_39862_WB_19011506_702.jpg
GTX101303_IHC_19011506_682.jpg
GTX101303_39862_WB_M_19011506_859.jpg
GTX101303_39862_20160525_IFA_19011506_248.jpg

Cat No. GTX101303

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

159 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , NCI-H929 , NIH-3T3 , JC , BCL-1
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BLM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Blm Recq Like Helicase,Bs,Recq2,Recql2,Recql3,Blm

Cellular Localization

Nucleus

Background

The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq]

Database

DATA IMAGES
GTX101303_39862_WB_19011506_702.jpg

GTX101303 WB Image

Sample (30 µg of whole cell lysate) 
  A: Jurkat
  B: Raji
  C: K562
  D: NCI-H929
  5% SDS PAGE 
  GTX101303 diluted at 1:1000 
 
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101303_IHC_19011506_682.jpg

GTX101303 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using BLM(GTX101303) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101303_39862_WB_M_19011506_859.jpg

GTX101303 WB Image

Sample (30 µg of whole cell lysate) 
        A: NIH-3T3
        B: JC
        C: BCL-1
        5% SDS PAGE 
        GTX101303 diluted at 1:1000
        
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101303_39862_20160525_IFA_19011506_248.jpg

GTX101303 ICC/IF Image

BLM antibody [C3], C-term detects BLM protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BLM protein stained by BLM antibody [C3], C-term (GTX101303) diluted at 1:200.
Blue: Hoechst 33342 staining.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169