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BLM antibody [N1N2], N-term

Anti-BLM antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101403
Anti-BLM antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101403
Anti-BLM antibody [N1N2], N-term used in Western Blot (WB). GTX101403
Anti-BLM antibody [N1N2], N-term used in Western Blot (WB). GTX101403
Anti-BLM antibody [N1N2], N-term used in Western Blot (WB). GTX101403

Cat. No. GTX101403

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BLM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

159 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BLM. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174690

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BLM RecQ like helicase , BS , MGRISCE1 , RECQ2 , RECQL2 , RECQL3

Cellular Localization

Nucleus

Background

The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities. Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity. The normal protein may act to suppress inappropriate recombination. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BLM antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101403

GTX101403 ICC/IF Image

BLM antibody [N1N2], N-term detects BLM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BLM stained by BLM antibody [N1N2], N-term (GTX101403) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-BLM antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101403

GTX101403 ICC/IF Image

BLM antibody [N1N2], N-term detects BLM protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BLM protein stained by BLM antibody [N1N2], N-term (GTX101403) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-BLM antibody [N1N2], N-term used in Western Blot (WB). GTX101403

GTX101403 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BLM antibody (GTX101403) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BLM antibody [N1N2], N-term used in Western Blot (WB). GTX101403

GTX101403 WB Image

BLM antibody detects BLM protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
BLM antibody (GTX101403) dilution: 1:5000

Anti-BLM antibody [N1N2], N-term used in Western Blot (WB). GTX101403

GTX101403 WB Image

BLM antibody detects BLM protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
5 % SDS-PAGE
BLM antibody (GTX101403) dilution: 1:5000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169