GeneTex
United States (US)

BLMH antibody [N1C1]

GTX102984_40562_WB_19011506_785.jpg

Cat No. GTX102984

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BLMH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BH antibody, BMH antibody, BLMH antibody, bleomycin hydrolase antibody, BLM hydrolase antibody

Cellular Localization

Cytoplasm

Background

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX102984_40562_WB_19011506_785.jpg

GTX102984 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX102984 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf