APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human BLNK expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
B cell linker , AGM4 , BASH , BLNK-S , LY57 , SLP-65 , SLP65 , bca
Cellular Localization
Cytoplasm,Cell membrane
Background
This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]
Database
Research Area
DATA IMAGES
![Anti-BLNK antibody [5G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60463](/templates/default/js/lightbox/images/loading.gif "Anti-BLNK antibody [5G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60463")
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GTX60463 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX60463 BLNK antibody [5G9].
Green : BLNK
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60463 WB Image
WB analysis of NIH3T3 (1) and BCBL-1 (2) cell lysate using GTX60463 BLNK antibody [5G9].
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GTX60463 ELISA Image
ELISA analysis of antigen using GTX60463 BLNK antibody [5G9].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
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GTX60463 FACS Image
FACS analysis of NIH3T3 cells using GTX60463 BLNK antibody [5G9].
Green : BLNK
Purple : negative control
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GTX60463 WB Image
WB analysis of human BLNK (AA: 34-216) recombinant protein using GTX60463 BLNK antibody [5G9].
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GTX60463 IHC-P Image
IHC-P analysis of human cervical cancer tissue using GTX60463 BLNK antibody [5G9].
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REFERENCE
There are currently no references for BLNK antibody [5G9] (GTX60463). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BLNK antibody [5G9] (GTX60463). Be the first to share your experience with this product.
![Anti-BLNK antibody [5G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60463](/upload/website/prouct_img/small/GTX60463/GTX60463_20170912_ICCIF_w_23061123_983.webp "Anti-BLNK antibody [5G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60463")
![Anti-BLNK antibody [5G9] used in Western Blot (WB). GTX60463](/upload/website/prouct_img/small/GTX60463/GTX60463_20170912_WB_1_w_23061123_409.webp "Anti-BLNK antibody [5G9] used in Western Blot (WB). GTX60463")
![Anti-BLNK antibody [5G9] used in ELISA (ELISA). GTX60463](/upload/website/prouct_img/small/GTX60463/GTX60463_20170912_ELISA_w_23061123_255.webp "Anti-BLNK antibody [5G9] used in ELISA (ELISA). GTX60463")
![Anti-BLNK antibody [5G9] used in Flow cytometry (FACS). GTX60463](/upload/website/prouct_img/small/GTX60463/GTX60463_20170912_FACS_w_23061123_120.webp "Anti-BLNK antibody [5G9] used in Flow cytometry (FACS). GTX60463")
![Anti-BLNK antibody [5G9] used in Western Blot (WB). GTX60463](/upload/website/prouct_img/small/GTX60463/GTX60463_20170912_WB_w_23061123_230.webp "Anti-BLNK antibody [5G9] used in Western Blot (WB). GTX60463")
![Anti-BLNK antibody [5G9] used in IHC (Paraffin sections) (IHC-P). GTX60463](/upload/website/prouct_img/small/GTX60463/GTX60463_20170912_IHC-P_w_23061123_749.webp "Anti-BLNK antibody [5G9] used in IHC (Paraffin sections) (IHC-P). GTX60463")
![Anti-BLNK antibody [5G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60463](/upload/website/prouct_img/normal/GTX60463/GTX60463_20170912_ICCIF_w_23061123_983.webp "Anti-BLNK antibody [5G9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60463")
![Anti-BLNK antibody [5G9] used in Western Blot (WB). GTX60463](/upload/website/prouct_img/normal/GTX60463/GTX60463_20170912_WB_1_w_23061123_409.webp "Anti-BLNK antibody [5G9] used in Western Blot (WB). GTX60463")
![Anti-BLNK antibody [5G9] used in ELISA (ELISA). GTX60463](/upload/website/prouct_img/normal/GTX60463/GTX60463_20170912_ELISA_w_23061123_255.webp "Anti-BLNK antibody [5G9] used in ELISA (ELISA). GTX60463")
![Anti-BLNK antibody [5G9] used in Flow cytometry (FACS). GTX60463](/upload/website/prouct_img/normal/GTX60463/GTX60463_20170912_FACS_w_23061123_120.webp "Anti-BLNK antibody [5G9] used in Flow cytometry (FACS). GTX60463")
![Anti-BLNK antibody [5G9] used in Western Blot (WB). GTX60463](/upload/website/prouct_img/normal/GTX60463/GTX60463_20170912_WB_w_23061123_230.webp "Anti-BLNK antibody [5G9] used in Western Blot (WB). GTX60463")
![Anti-BLNK antibody [5G9] used in IHC (Paraffin sections) (IHC-P). GTX60463](/upload/website/prouct_img/normal/GTX60463/GTX60463_20170912_IHC-P_w_23061123_749.webp "Anti-BLNK antibody [5G9] used in IHC (Paraffin sections) (IHC-P). GTX60463")