APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89949-PEP).
Product Note
This antibody is expected to recognize both reported isoforms (NP_037446.1; NP_001107566.1).
Predict Reactivity
Mouse, Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1; NP_001107566.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
B cell linker , AGM4 , BASH , BLNK-S , LY57 , SLP-65 , SLP65 , bca
Cellular Localization
Cytoplasm , Cell membrane
Background
This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]
Database
Research Area
DATA IMAGES
|
GTX89949 WB Image
WB analysis of HEK293 overexpressing BLNK (mock transfection in first lane) using GTX89949 BLNK antibody, C-term.
|
|
|
GTX89949 WB Image
WB analysis of Daudi lysate using GTX89949 BLNK antibody, C-term.
Dilution : 4μg/ml
Loading : 30μg protein in RIPA buffer
|
REFERENCE
There are currently no references for BLNK antibody, C-term (GTX89949). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BLNK antibody, C-term (GTX89949). Be the first to share your experience with this product.
